keratinocyte growth Search Results


keratinocyte growth kit  (ATCC)
96
ATCC keratinocyte growth kit
Expression of HSP47, HSP90, TGF-β, and DEJ proteins following RF treatment in senescent <t>keratinocytes.</t> ( A ) Schematic representation of non-senescent keratinocytes, senescent keratinocytes, and RF treatments administered to senescent keratinocytes. ( B – H ) Proteins were extracted from senescent keratinocytes following RF treatment and subjected to the following assays. ( B – D ) Western blot analysis of HSP47 and HSP90. ( E ) ELISA results for TGF-β. ( F , G ) Western blot analysis of collagen XVII. ( H ) ELISA results for collagen IV. Data are presented as mean ± SD of three independent experiments. **, p < 0.01 vs. first bar; $$, p < 0.01 vs. second bar (Mann–Whitney U test). CON, control; DEJ, dermal–epidermal junction; ELISA, enzyme-linked immunosorbent assay; GM, growth medium; HSP47, heat shock protein 47; HSP90, heat shock protein 90; MW, molecular weight; PBS, phosphate-buffered saline; RF, radiofrequency; SD, standard deviation; SnCs, senescent; TGF-β, transforming growth factor beta.
Keratinocyte Growth Kit, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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keratinocyte growth kit components  (ATCC)
95
ATCC keratinocyte growth kit components
Expression of HSP47, HSP90, TGF-β, and DEJ proteins following RF treatment in senescent <t>keratinocytes.</t> ( A ) Schematic representation of non-senescent keratinocytes, senescent keratinocytes, and RF treatments administered to senescent keratinocytes. ( B – H ) Proteins were extracted from senescent keratinocytes following RF treatment and subjected to the following assays. ( B – D ) Western blot analysis of HSP47 and HSP90. ( E ) ELISA results for TGF-β. ( F , G ) Western blot analysis of collagen XVII. ( H ) ELISA results for collagen IV. Data are presented as mean ± SD of three independent experiments. **, p < 0.01 vs. first bar; $$, p < 0.01 vs. second bar (Mann–Whitney U test). CON, control; DEJ, dermal–epidermal junction; ELISA, enzyme-linked immunosorbent assay; GM, growth medium; HSP47, heat shock protein 47; HSP90, heat shock protein 90; MW, molecular weight; PBS, phosphate-buffered saline; RF, radiofrequency; SD, standard deviation; SnCs, senescent; TGF-β, transforming growth factor beta.
Keratinocyte Growth Kit Components, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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normal human epidermal keratinocytes  (PromoCell)
97
PromoCell normal human epidermal keratinocytes
Expression of HSP47, HSP90, TGF-β, and DEJ proteins following RF treatment in senescent <t>keratinocytes.</t> ( A ) Schematic representation of non-senescent keratinocytes, senescent keratinocytes, and RF treatments administered to senescent keratinocytes. ( B – H ) Proteins were extracted from senescent keratinocytes following RF treatment and subjected to the following assays. ( B – D ) Western blot analysis of HSP47 and HSP90. ( E ) ELISA results for TGF-β. ( F , G ) Western blot analysis of collagen XVII. ( H ) ELISA results for collagen IV. Data are presented as mean ± SD of three independent experiments. **, p < 0.01 vs. first bar; $$, p < 0.01 vs. second bar (Mann–Whitney U test). CON, control; DEJ, dermal–epidermal junction; ELISA, enzyme-linked immunosorbent assay; GM, growth medium; HSP47, heat shock protein 47; HSP90, heat shock protein 90; MW, molecular weight; PBS, phosphate-buffered saline; RF, radiofrequency; SD, standard deviation; SnCs, senescent; TGF-β, transforming growth factor beta.
Normal Human Epidermal Keratinocytes, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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keratinocyte growth medium 2  (PromoCell)
97
PromoCell keratinocyte growth medium 2
Expression of HSP47, HSP90, TGF-β, and DEJ proteins following RF treatment in senescent <t>keratinocytes.</t> ( A ) Schematic representation of non-senescent keratinocytes, senescent keratinocytes, and RF treatments administered to senescent keratinocytes. ( B – H ) Proteins were extracted from senescent keratinocytes following RF treatment and subjected to the following assays. ( B – D ) Western blot analysis of HSP47 and HSP90. ( E ) ELISA results for TGF-β. ( F , G ) Western blot analysis of collagen XVII. ( H ) ELISA results for collagen IV. Data are presented as mean ± SD of three independent experiments. **, p < 0.01 vs. first bar; $$, p < 0.01 vs. second bar (Mann–Whitney U test). CON, control; DEJ, dermal–epidermal junction; ELISA, enzyme-linked immunosorbent assay; GM, growth medium; HSP47, heat shock protein 47; HSP90, heat shock protein 90; MW, molecular weight; PBS, phosphate-buffered saline; RF, radiofrequency; SD, standard deviation; SnCs, senescent; TGF-β, transforming growth factor beta.
Keratinocyte Growth Medium 2, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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keratinocyte growth medium 2 kit  (PromoCell)
95
PromoCell keratinocyte growth medium 2 kit
Expression of HSP47, HSP90, TGF-β, and DEJ proteins following RF treatment in senescent <t>keratinocytes.</t> ( A ) Schematic representation of non-senescent keratinocytes, senescent keratinocytes, and RF treatments administered to senescent keratinocytes. ( B – H ) Proteins were extracted from senescent keratinocytes following RF treatment and subjected to the following assays. ( B – D ) Western blot analysis of HSP47 and HSP90. ( E ) ELISA results for TGF-β. ( F , G ) Western blot analysis of collagen XVII. ( H ) ELISA results for collagen IV. Data are presented as mean ± SD of three independent experiments. **, p < 0.01 vs. first bar; $$, p < 0.01 vs. second bar (Mann–Whitney U test). CON, control; DEJ, dermal–epidermal junction; ELISA, enzyme-linked immunosorbent assay; GM, growth medium; HSP47, heat shock protein 47; HSP90, heat shock protein 90; MW, molecular weight; PBS, phosphate-buffered saline; RF, radiofrequency; SD, standard deviation; SnCs, senescent; TGF-β, transforming growth factor beta.
Keratinocyte Growth Medium 2 Kit, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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growth medium  (PromoCell)
94
PromoCell growth medium
Expression of HSP47, HSP90, TGF-β, and DEJ proteins following RF treatment in senescent <t>keratinocytes.</t> ( A ) Schematic representation of non-senescent keratinocytes, senescent keratinocytes, and RF treatments administered to senescent keratinocytes. ( B – H ) Proteins were extracted from senescent keratinocytes following RF treatment and subjected to the following assays. ( B – D ) Western blot analysis of HSP47 and HSP90. ( E ) ELISA results for TGF-β. ( F , G ) Western blot analysis of collagen XVII. ( H ) ELISA results for collagen IV. Data are presented as mean ± SD of three independent experiments. **, p < 0.01 vs. first bar; $$, p < 0.01 vs. second bar (Mann–Whitney U test). CON, control; DEJ, dermal–epidermal junction; ELISA, enzyme-linked immunosorbent assay; GM, growth medium; HSP47, heat shock protein 47; HSP90, heat shock protein 90; MW, molecular weight; PBS, phosphate-buffered saline; RF, radiofrequency; SD, standard deviation; SnCs, senescent; TGF-β, transforming growth factor beta.
Growth Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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growth medium - by Bioz Stars, 2026-03
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fgf10  (MedChemExpress)
94
MedChemExpress fgf10
Expression of HSP47, HSP90, TGF-β, and DEJ proteins following RF treatment in senescent <t>keratinocytes.</t> ( A ) Schematic representation of non-senescent keratinocytes, senescent keratinocytes, and RF treatments administered to senescent keratinocytes. ( B – H ) Proteins were extracted from senescent keratinocytes following RF treatment and subjected to the following assays. ( B – D ) Western blot analysis of HSP47 and HSP90. ( E ) ELISA results for TGF-β. ( F , G ) Western blot analysis of collagen XVII. ( H ) ELISA results for collagen IV. Data are presented as mean ± SD of three independent experiments. **, p < 0.01 vs. first bar; $$, p < 0.01 vs. second bar (Mann–Whitney U test). CON, control; DEJ, dermal–epidermal junction; ELISA, enzyme-linked immunosorbent assay; GM, growth medium; HSP47, heat shock protein 47; HSP90, heat shock protein 90; MW, molecular weight; PBS, phosphate-buffered saline; RF, radiofrequency; SD, standard deviation; SnCs, senescent; TGF-β, transforming growth factor beta.
Fgf10, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse keratinocyte growth factor elisa kit  (Cusabio)
91
Cusabio mouse keratinocyte growth factor elisa kit
Expression of HSP47, HSP90, TGF-β, and DEJ proteins following RF treatment in senescent <t>keratinocytes.</t> ( A ) Schematic representation of non-senescent keratinocytes, senescent keratinocytes, and RF treatments administered to senescent keratinocytes. ( B – H ) Proteins were extracted from senescent keratinocytes following RF treatment and subjected to the following assays. ( B – D ) Western blot analysis of HSP47 and HSP90. ( E ) ELISA results for TGF-β. ( F , G ) Western blot analysis of collagen XVII. ( H ) ELISA results for collagen IV. Data are presented as mean ± SD of three independent experiments. **, p < 0.01 vs. first bar; $$, p < 0.01 vs. second bar (Mann–Whitney U test). CON, control; DEJ, dermal–epidermal junction; ELISA, enzyme-linked immunosorbent assay; GM, growth medium; HSP47, heat shock protein 47; HSP90, heat shock protein 90; MW, molecular weight; PBS, phosphate-buffered saline; RF, radiofrequency; SD, standard deviation; SnCs, senescent; TGF-β, transforming growth factor beta.
Mouse Keratinocyte Growth Factor Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human fgf  (Proteintech)
93
Proteintech recombinant human fgf
Expression of HSP47, HSP90, TGF-β, and DEJ proteins following RF treatment in senescent <t>keratinocytes.</t> ( A ) Schematic representation of non-senescent keratinocytes, senescent keratinocytes, and RF treatments administered to senescent keratinocytes. ( B – H ) Proteins were extracted from senescent keratinocytes following RF treatment and subjected to the following assays. ( B – D ) Western blot analysis of HSP47 and HSP90. ( E ) ELISA results for TGF-β. ( F , G ) Western blot analysis of collagen XVII. ( H ) ELISA results for collagen IV. Data are presented as mean ± SD of three independent experiments. **, p < 0.01 vs. first bar; $$, p < 0.01 vs. second bar (Mann–Whitney U test). CON, control; DEJ, dermal–epidermal junction; ELISA, enzyme-linked immunosorbent assay; GM, growth medium; HSP47, heat shock protein 47; HSP90, heat shock protein 90; MW, molecular weight; PBS, phosphate-buffered saline; RF, radiofrequency; SD, standard deviation; SnCs, senescent; TGF-β, transforming growth factor beta.
Recombinant Human Fgf, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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keratinocyte  (MedChemExpress)
94
MedChemExpress keratinocyte
Expression of HSP47, HSP90, TGF-β, and DEJ proteins following RF treatment in senescent <t>keratinocytes.</t> ( A ) Schematic representation of non-senescent keratinocytes, senescent keratinocytes, and RF treatments administered to senescent keratinocytes. ( B – H ) Proteins were extracted from senescent keratinocytes following RF treatment and subjected to the following assays. ( B – D ) Western blot analysis of HSP47 and HSP90. ( E ) ELISA results for TGF-β. ( F , G ) Western blot analysis of collagen XVII. ( H ) ELISA results for collagen IV. Data are presented as mean ± SD of three independent experiments. **, p < 0.01 vs. first bar; $$, p < 0.01 vs. second bar (Mann–Whitney U test). CON, control; DEJ, dermal–epidermal junction; ELISA, enzyme-linked immunosorbent assay; GM, growth medium; HSP47, heat shock protein 47; HSP90, heat shock protein 90; MW, molecular weight; PBS, phosphate-buffered saline; RF, radiofrequency; SD, standard deviation; SnCs, senescent; TGF-β, transforming growth factor beta.
Keratinocyte, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fgf7  (MedChemExpress)
93
MedChemExpress fgf7
Final concentration of differentiation factors.
Fgf7, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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epidermal keratinocytes  (PromoCell)
93
PromoCell epidermal keratinocytes
Final concentration of differentiation factors.
Epidermal Keratinocytes, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Expression of HSP47, HSP90, TGF-β, and DEJ proteins following RF treatment in senescent keratinocytes. ( A ) Schematic representation of non-senescent keratinocytes, senescent keratinocytes, and RF treatments administered to senescent keratinocytes. ( B – H ) Proteins were extracted from senescent keratinocytes following RF treatment and subjected to the following assays. ( B – D ) Western blot analysis of HSP47 and HSP90. ( E ) ELISA results for TGF-β. ( F , G ) Western blot analysis of collagen XVII. ( H ) ELISA results for collagen IV. Data are presented as mean ± SD of three independent experiments. **, p < 0.01 vs. first bar; $$, p < 0.01 vs. second bar (Mann–Whitney U test). CON, control; DEJ, dermal–epidermal junction; ELISA, enzyme-linked immunosorbent assay; GM, growth medium; HSP47, heat shock protein 47; HSP90, heat shock protein 90; MW, molecular weight; PBS, phosphate-buffered saline; RF, radiofrequency; SD, standard deviation; SnCs, senescent; TGF-β, transforming growth factor beta.

Journal: International Journal of Molecular Sciences

Article Title: Radiofrequency Treatment Attenuates Age-Related Changes in Dermal–Epidermal Junctions of Animal Skin

doi: 10.3390/ijms25105178

Figure Lengend Snippet: Expression of HSP47, HSP90, TGF-β, and DEJ proteins following RF treatment in senescent keratinocytes. ( A ) Schematic representation of non-senescent keratinocytes, senescent keratinocytes, and RF treatments administered to senescent keratinocytes. ( B – H ) Proteins were extracted from senescent keratinocytes following RF treatment and subjected to the following assays. ( B – D ) Western blot analysis of HSP47 and HSP90. ( E ) ELISA results for TGF-β. ( F , G ) Western blot analysis of collagen XVII. ( H ) ELISA results for collagen IV. Data are presented as mean ± SD of three independent experiments. **, p < 0.01 vs. first bar; $$, p < 0.01 vs. second bar (Mann–Whitney U test). CON, control; DEJ, dermal–epidermal junction; ELISA, enzyme-linked immunosorbent assay; GM, growth medium; HSP47, heat shock protein 47; HSP90, heat shock protein 90; MW, molecular weight; PBS, phosphate-buffered saline; RF, radiofrequency; SD, standard deviation; SnCs, senescent; TGF-β, transforming growth factor beta.

Article Snippet: Primary human epidermal keratinocytes (HEKn; American Type Culture Collection [ATCC], Manassas, VA, USA) were cultured in growth medium (GM) composed of dermal cell basal medium (ATCC) supplemented with a keratinocyte growth kit (ATCC).

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY, Control, Molecular Weight, Saline, Standard Deviation

Final concentration of differentiation factors.

Journal: Frontiers in Cell and Developmental Biology

Article Title: A differentiation protocol for generating pancreatic delta cells from human pluripotent stem cells

doi: 10.3389/fcell.2024.1490040

Figure Lengend Snippet: Final concentration of differentiation factors.

Article Snippet: • Activin A (StemCell Technologies, cat. no. 78001) • CHIR99021 (Stemgent, cat. no. 04-0004-10) • FGF7 (StemCell, cat. no. 78046) • FGF2 (Med Chem Express, cat. no. HY-P7004) • Retinoic acid (Sigma, cat. no. R2625) • SANT-1 (Sigma, cat. no. S4572) • LDN193189 (Reprocell, cat. no. 40074) • PdBU (Millipore, cat. no. 524390) • ALK5i II (Cell Guidance Systems, cat. no. SM09-50) • XXI (Millipore, cat. no. 595790) • Betacellulin (Med Chem Express, cat. no. HY-P7005) • Heparin (Sigma, cat. no. H3149-500KU) • N-acetyl cysteine (Sigma, cat. no. A9165-100G) • R428 (Selleck, cat. no. S2841) • α-Tocopherol (Sigma, cat. no. T3251) • Zinc sulfate (Sigma, cat. no. Z0251) • PBS for dissolving factors (HyClone, cat. no. SH30256.01) • Dimethyl sulfoxide for dissolving factors (DMSO) (MilliporeSigma, cat. no. D4540-100ML)

Techniques: Concentration Assay